Sunday, April 4, 2010

MICROBIOLOGY

MICRO BIOLOGY EAMCET QUESTION PAPER

BOTANY
1. The chemicals used in Gram staining process are obtained from
1) Clostridium acetobutylium and lactobacillus
2) Acetobacter aceti and zymomonas mobilis
3) Clostridium acetobutylicom and zymomonas mobilis
4) Zymomonas mobilis and bacillus pollymyxa

2. In the following diseases how many are not caused by bacteria
I. Dysentry II. Influenza III. Polio IV. Plaque
v. Syphilis VI. AIDS VII. Vibriosis
1) 2 2) 3 3) 4 4) 5

3. Arrange the following in an ascending order that are involved during the uptake of a naked DNA molecule by a bacterial cell to achieve genetic recombination
I. Endonucleases II. Competence specific protein
III. Nucleases IV. DNA binding protein
1) I, III, IV, II 2) IV, I, III, II 3) IV, I, II, III 4) I, IV, III, II

4. Assertion (A): Corynebacterium renale has fimbriae.
Reason (R): The presence of fimbriae is the main feature of gram –ve bacteria..
1) Both A and R are true R is the correct explanation to the A
2) Both A and R are true R is not correct explanation to the A
3) A is true but R is false 4) A is false but R is true

5. Assertion (A): The genetic material of maize rough dwarf virus is ssRNA.
Reason (R): Most of the plant viruses contains ssRNA as genetic material.
1) Both A and R are true R is the correct explanation to the A
2) Both A and R are true R is not correct explanation to the A
3) A is true but R is false 4) A is false but R is true

6. Study the following table and find out the incorrect combination
I. Beggiota Filamentous Oxidises H2S
II. Bdellovibrio Parasite Insects
III. Corynebacterium renale Gram +ve Lysine
IV. Thiomargarita Coccus Atrichous
1) I and III 2) II and III 3) I and IV 4) II and IV

7. In the following kingdoms how many are includes the microorganisms
1) Animalia II. Fungi III. Monera IV. Protista V. Plantae
1) 2 2) 3 3) 4 4) 1

8. The maximum length of longest rod shaped bacterium is how many times greater than the minimum average length of other bacteria
1) 100 times 2) 500 times 3) 250 times 4) 350 times

9. The largest halophilic bacteria is found in the form of
1) Monococcus 2) Strepto coccus 3) Diplo coccus 4) Sarcina

10. Which of the following shapes are not exhibited by acetobacter
A) Oval B) Small rods C) Ellipsoidal D) Chain of cocci
E) Chain of bacilli
1) A and E 2) C and D 3) A and D 4) C and E

11. Comparatively the structure present out side to the cell wall is not essential for bacterial survival is
1) slime layer 2) s-layer 3) capsule 4) pili

12. The ratio between no. of protein filaments present in a flagellum of bacterium and flagellum of a eukaryotic cell
1) 1 : 11 2) 3 : 9 3) 1 : 3 4) 3 : 11

13. What are following structures that are very essential for the lithophytic bacteria for fixation
A) Capsule B) Slime layer C) S-layer D) Flagella
E) Pili
1) A and E 2) B and C 3) C and D 4) B and E

14. Find out the incorrect statements
A. All gram –ve bacteria contains pili
B. All gram +ve bacteria contains teichoic acid
C. Minimum no. of flagella present in amphitrichous bacterium is two
D. Sex pili are more in number than fimbriae
E. Sex pili are smaller than fimbriae and having hollow core
1) All except two 2) All except one 3) All except three 4) All

15. A. H2 + O2 ® H2O B. S ® H2SO4 C. NH3 ® NO2–
D. Fe++ ® Fe+++ E. H2S ® S
In the above reaction what are the reactions oxidized by the rod shaped bacteria
1) A and D 2) B and C 3) B and D 4) C and E

16. How many of the following bacteria having chlorophylls
A. Hydrogenomonas B. Rhodospirillum C. Chlorobium D. Nitrobacter
E. Rhodo microbium F. Chromatium
1) 2 2) 3 3) 5 4) 4

17. Find out the bacteria that are involved in the following reactions
1) N2 NH3 NO3– N2
1) A – Nostoc, anabaena B – Nitrobacter, nitrosomonas C – Thiobacillus
2) A – Rhizobium, Azospirillum B – Bacillus, Clostridium C – Azotobacter
3) A – Rhizobium, Azospirillum B – Nitrosomonas, Nitrobacter C – thio bacillus
4) Rhizobium, Azospirillum B – Bacillus, Azotobacter C – Nostoc, Anabaence

18. Endospore is surrounded by how many protein layers?
1) 3 2) 4 3) 2 4) Many

19. Assertion (A): In HFR x F– conjugation F– cell does not become Donor cell.
Reason (R): In this conjugation genetic material is not transferred from HFR to F–.
1) Both A and R are true R is the correct explanation to the A
2) Both A and R are true R is not correct explanation to the A
3) A is true but R is false 4) A is false but R is true

20. Which of the following antibiotics are not obtained from streptomyces sps?
A. Oxytetra cyclin B. Bacitracin C. Vinegar D. Neomycin
E. Polymyxin B F. Chloram phenical F. Acetone G. Amphotericon
1) A D E G 2) B C E F 3) B E only 4) C F only

21. Find out the incorrect combinations
Disease Host Bacterium
A. Tuberculosis Dog Mycobacterium tuberculosis
B. Crown gall Apple Erwinia amylovora
C. Diphtheria Man Bacillus diphtheriae
D. Actinomycosis Cattle Vibrio tetus
E. Fire blight Pear Agrobacterium
1) All 2) All except two 3) All except one 4) All except three

22. What are biochemical techniques used to prove that the genetic material in TMV is RNA?
A. Ultracentrifugation B. Microchemical tests
C. X-ray crystallography D. Chromato graphy
E. Gel electrophoresis F. Southern blotting
G. Electron microspopy
1) A E F G 2) B D E G 3) A C G 4) C E G

23. Find out the correct statements?
A. Bacteriophages contains dsDNA or ssDNA or dsRNA
B. Phytophages contains dsDNA or ssRNA or dsRNA
C. Zoophages contains, dsDNA or dsRNA
D. All animal viruses contains DNA
E. Genetic material of host of phytophage is always DNA
F. Genetic material of host of zymophage is always RNA
1) All are correct 2) All except two 3) All except three 4) All except four

24. T2 bacteriophage shows how many equilateral triangle faces
1) 20 faces in head and 20 faces in tail 2) 20 faces in head and many faces in tail
3) Many faces in head and 20 faces in tail 4) 20 faces in head and no triangle faces in tail

25. How many nucleotides are present in the mRNA molecule which is involved in production of a single capsomere of TMV?
1) 158 2) 477 3) 474 4) 316

26. Arrange the following measurements in a descending order
A. Length of TMV B. Length of T2 bacteriophage
C. Diameter of TMV 4) Length of tail of bacteriophage
E. Diameter of central hole of TMV
1) A B C E D 2) A B D C E 3) E C D B C 4) D E C B A

27. The approximate length of T2 bacteriophage DNA is
1) 95m 2) 65m 3) 190m 4) 1000m

28. The term vegetative phage is applicable to
A. Temperature phage B. Virulent phage
C. Lysogen D. Lytic phage
1) A B C 2) A B D 3) B C D 4) A C D

29. The following processes are takes place in how many phases?
A. Lysozyme action B. Host DNA degradasion
C. Collision between viron and bacteriophages D. Tail fibres usage
E. Host cell wall ruptures
1) 1 2) 4 3) 2 4) 3

30. Mosaic diseases caused by viruses are transmitted through
A. Seeds B. Pollengrains C. Insects D. Fungi
E. mechanical transmission F. Nematodes
1) A B D E 2) A C E 3) B D F 4) A B D F

31. Assertion (A): Vector control is considered as an important strategy in preventing viral diseases.
Reason (R): Most of the plant viruses are transmitted by air, wind and water.
1) Both A and R are true R is the correct explanation to the A
2) Both A and R are true R is not correct explanation to the A
3) A is true but R is false 4) A is false but R is true

32. Which of the following disease is transmitted by non-motile spores
1) Tobacco necrosis disease 2) Lettuce mosaic disease
3) Cherry ring spot disease 4) Potato leaf roll



33. Assertion (A): Transduction is occurs very rare in nature.
Reason (R): Rarely 2 or 3 in one million phages take part in lysogenic cycle.
1) Both A and R are true R is the correct explanation to the A
2) Both A and R are true R is not correct explanation to the A
3) A is true but R is false 4) A is false but R is true

34. A DNA molecule of T2 bacteriophage is made up of 300 nucleotides out of which 60 are 2-amino oxypyrines. What will be the ratio of 6-amino purines and 5-hydroxy methyl pyrimidines seen in this
1) 1 : 1 2) 2 : 3 3) 3 : 2 4) 2 : 1

35. A bacterium divides once in every 35 minutes. If a culture containing 105 cells per ml is grown for 3 hrs short of 5 minutes. What will be the cell concentration per ml.?
1) 30 x 105 cells 2) 32 x 105 cells 3) 10 x 105 cells 4) 175 x 105 cells

36. Following is incorrect statement regarding Beggiota
A. It’s a filament like
B. Produce elemental sulphur during oxidation
C. It derives energy from organic substance
D. It oxidizes hydrogen in the presence of oxygen
1) A C 2) A D 3) C D 4) A B

37. Assertion (A): In lysogenic life cycle the host cell does not get lysed immediately.
Reason (R): Viral DNA integrated with bacterial genome.
1) Both A and R are true R is the correct explanation to the A
2) Both A and R are true R is not correct explanation to the A
3) A is true but R is false 4) A is false but R is true

38. Which of the following is not true about plant diseases caused by phytophages.
I. The plant diseases by viruses are generally systemic.
II. Symptoms of the diseases generally observed in leaves
III. Breaking of flowers in seen in coco
IV. Vein banding seen in Bhendi
1) I, III 2) III, IV 3) II, IV 4) I, IV

39. There are 8 sarcina on a laboratory slide. Total number of chromosomes found on slide is
1) 64 2) 8 3) 32 4) 16

40. The approximate length of RNA molecule of TMV is
1) 6500Å 2) 22100Å 3) 39 x 106 Å 4) 22100 nm
KEY
1)
3
2)
2
3)
2
4)
2
5)
4
6)
2
7)
3
8)
3
9)
2
10)
3
11)
3
12)
4
13)
4
14)
1
15)
3
16)
4
17)
3
18)
4
19)
3
20)
3
21)
3
22)
3
23)
2
24)
4
25)
2
26)
2
27)
3
28)
2
29)
4
30)
2
31)
3
32)
3
33)
3
34)
3
35)
2
36)
3
37)
1
38)
2
39)
1
40)
2

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